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+Incubate the membrane for 20 min after which wash with DMF to remove uncoupled amino acids. Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:one hundred dilution. Note that the facet chain has three methylene teams, so that despite the fact that the terminal amino group might be charged beneath physiological conditions, the aspect chain does have important hydrophobic character.
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